You Are Reading

Research: Establishment of a biobank for human lung tissues of congenital diaphragmatic hernia and congenital pulmonary airway malformation

0
Uncategorized

Research: Establishment of a biobank for human lung tissues of congenital diaphragmatic hernia and congenital pulmonary airway malformation

J Pediatr Surg. 2019 May 11. pii: S0022-3468(19)30346-X. doi: 10.1016/j.jpedsurg.2019.05.003. [Epub ahead of print]

https://www.ncbi.nlm.nih.gov/pubmed/31130348

Establishment of a biobank for human lung tissues of congenital diaphragmatic hernia and congenital pulmonary airway malformation.

Wagner R1Ayoub L2Kahnamoui S2Li H2Patel D2Liu D2Del Bigio MR3Stefanovici C3Lacher M4Keijzer R5.

Author information

Abstract

BACKGROUND:

Human tissue samples are an invaluable and little available source of information for translational studies of congenital lung diseases such as Congenital Diaphragmatic Hernia (CDH) or Congenital Pulmonary Airway Malformation (CPAM).

PURPOSE:

We aimed to establish a human lung tissue biobank of CDH and CPAM patients together with age-matched controls, coupled with a clinical database.

METHODS:

Pathology records from autopsies or surgical specimens for CDH and CPAM cases between 1980 and 2017 were reviewed. For surviving individuals, clinical patient data was obtained from corresponding pediatric surgery reports. Formalin-fixed, paraffin-embedded tissues of patients and age-matched controls were systematically stored for further translational studies. RNA integrity was determined on selected CDH blocks.

RESULTS:

A total of 16 CDH and 18 CPAM and age-matched control lung tissue blocks were included in our biobank. Ages ranged from 22 to 41 weeks of gestation (GA) in CDH (33.9 ± 6.35 weeks) and 26 weeks (GA) and 12 years in CPAM (2.3 ± 3.7 y). RNA isolation from CDH and control blocks yielded good RNA quality (OD 260/280 ratio: 2.01-2.09, OD 260/230 ratio: 2.04-2.09).

CONCLUSION:

We established a unique human biobank for CDH and CPAM tissues. The combination with clinical patient data will allow us to design future translational studies to improve our understanding of the disease pathogenesis of these congenital malformations.

Copyright © 2019. Published by Elsevier Inc.

KEYWORDS:

Biobank; Congenital diaphragmatic hernia; Congenital lung anomalies; Congenital pulmonary airway malformation; Human lung tissuePMID: 31130348 DOI: 10.1016/j.jpedsurg.2019.05.003

Leave a Reply

Your email address will not be published. Required fields are marked *

Follow @ Instagram