J Tissue Eng Regen Med
. 2020 Nov 11. doi: 10.1002/term.3151. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/33175464/
Design and fabrication of polycaprolactone/gelatin composite scaffolds for diaphragmatic muscle reconstruction
Tina Navaei 1 2, Peiman Brouki Milan 1 2, Ali Samadikuchaksaraei 1 2, Hamid Reza Davari 3, John G Hardy 4, Masoud Mozafari 1 2Affiliations expand
- PMID: 33175464
- DOI: 10.1002/term.3151
Abstract
Diaphragmatic wall defects caused by congenital disorders or disease remain a major challenge for physicians worldwide. Polymeric patches have been extensively explored within research laboratories and the clinic for soft tissue and diaphragm reconstruction. However, patch usage is usually associated with allergic reaction, infection, granulation, and recurrence of the hernia. In this study, we designed and fabricated a porous scaffold using 3D printing and freeze-drying techniques. A polycaprolactone (PCL) mesh, as a reinforcement material, offers the scaffolds an advantage over previously reported examples since it provides mechanical strength and flexibility. The results showed that adherent cells were anchorage-dependent and grew as a monolayer attached to the scaffolds. Microscopic observations indicated better cell attachments for the scaffolds with higher gelatin content as compared with the control sample. According to tensile test results, the mechanical strength of samples was significantly greater than adult diaphragm tissue. The biocompatibility of the specimens was investigated in vivo using a subcutaneous implantation method in five BALB/c adult mice for 20 days, with the results indicating superior cellular behavior and attachment of the fibroblasts on scaffolds containing gelatin in comparison to pure PCL scaffolds. The proposed porous PCL/gelatin scaffolds show promise as potential biodegradable and flexible constructs for diaphragm reconstruction. This article is protected by copyright. All rights reserved.
Keywords: Biomaterials; Cellular response; Diaphragm; Regeneration; Scaffold; Tissue engineering.
This article is protected by copyright. All rights reserved.
