Front Pediatr. 2019 Nov 22;7:490. doi: 10.3389/fped.2019.00490. eCollection 2019. https://www.ncbi.nlm.nih.gov/pubmed/31824902
Endothelial Progenitor and Mesenchymal Stromal Cells in Newborns With Congenital Diaphragmatic Hernia Undergoing Extracorporeal Membrane Oxygenation.
Rafat N1,2,3, Patry C2, Sabet U2, Viergutz T4, Weiss C5, Tönshoff B2, Beck G6, Schaible T1.
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Abstract
Background: Endothelial progenitor (EPC) and mesenchymal stromal cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. We do not know, how extracorporeal membrane oxygenation (ECMO) might affect EPC- and MSC-mediated regenerative pathways in patients with congenital diaphragmatic hernia (CDH). Therefore, we investigated, if ECMO support impacts EPC and MSC numbers in CDH patients. Methods: Peripheral blood mononuclear cells from newborns with ECMO-dependent (n = 18) and ECMO-independent CDH (n = 12) and from healthy controls (n = 12) were isolated. The numbers of EPC and MSC were identified by flowcytometry. Serum levels of vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-2 were determined. Results: EPC and MSC were elevated in newborns with CDH. ECMO-dependent infants had higher EPC subpopulation counts (2,1-7,6-fold) before treatment compared to ECMO-independent infants. In the disease course, EPC and MSC subpopulation counts in ECMO-dependent infants were lower than before ECMO initiation. During ECMO, VEGF serum levels were significantly reduced (by 90.5%) and Ang2 levels significantly increased (by 74.8%). Conclusions: Our data suggest that ECMO might be associated with a rather impaired mobilization of EPC and MSC and with a depression of VEGF serum levels in newborns with CDH.
Copyright © 2019 Rafat, Patry, Sabet, Viergutz, Weiss, Tönshoff, Beck and Schaible.
KEYWORDS:
ECMO; congenital diaphragmatic hernia; neonates; progenitor cells; stem cellsPMID: 31824902 PMCID: PMC6882772 DOI: 10.3389/fped.2019.00490
