Cell Rep Methods
. 2023 Oct 4:100613.
doi: 10.1016/j.crmeth.2023.100613. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/37827157/
A pneumonectomy model to study flow-induced pulmonary hypertension and compensatory lung growth
Savas T Tsikis 1, Timothy Klouda 2, Thomas I Hirsch 1, Scott C Fligor 1, Tiffany Liu 2, Yunhye Kim 2, Amy Pan 1, Mikayla Quigley 1, Paul D Mitchell 3, Mark Puder 4, Ke Yuan 5
Affiliations expand
- PMID: 37827157
- DOI: 10.1016/j.crmeth.2023.100613
Abstract
In newborns, developmental disorders such as congenital diaphragmatic hernia (CDH) and specific types of congenital heart disease (CHD) can lead to defective alveolarization, pulmonary hypoplasia, and pulmonary arterial hypertension (PAH). Therapeutic options for these patients are limited, emphasizing the need for new animal models representative of disease conditions. In most adult mammals, compensatory lung growth (CLG) occurs after pneumonectomy; however, the underlying relationship between CLG and flow-induced pulmonary hypertension (PH) is not fully understood. We propose a murine model that involves the simultaneous removal of the left lung and right caval lobe (extended pneumonectomy), which results in reduced CLG and exacerbated reproducible PH. Extended pneumonectomy in mice is a promising animal model to study the cellular response and molecular mechanisms contributing to flow-induced PH, with the potential to identify new treatments for patients with CDH or PAH-CHD.
Keywords: CP: Biotechnology; animal models; compensatory lung growth; pneumonectomy; pulmonary hypertension; pulmonary hypoplasia.
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
