fbpx
You Are Reading

Research: Tenascin C is dysregulated in hypoplastic lungs of miR-200b -/- mice

0
Uncategorized

Research: Tenascin C is dysregulated in hypoplastic lungs of miR-200b -/- mice

Pediatr Surg Int

. 2022 Mar 2. doi: 10.1007/s00383-022-05096-0. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/35235015/

Tenascin C is dysregulated in hypoplastic lungs of miR-200b -/- mice

Moritz Markel 1 2Wai Hei Tse 1Nolan DeLeon 1Daywin Patel 1Shana Kahnamouizadeh 1Martin Lacher 2Richard Wagner 2Richard Keijzer 3Affiliations expand

Abstract

Purpose: We previously demonstrated that absence of miR-200b results in abnormal lung development in congenital diaphragmatic hernia due to imbalance between epithelial and mesenchymal cells. Tenascin C is a highly conserved extracellular matrix protein involved in epithelial to mesenchymal transition, tissue regeneration and lung development. Considering the involvement of Tenascin C and miR-200b and their potential interaction, we aimed to study Tenascin C during lung development in the absence of miR-200b.

Methods: We collected lungs of miR-200b-/- mice (male, 8 weeks). We performed Western blot (WB) analysis (N = 6) and immunofluorescence (N = 5) for Tenascin C and alpha smooth muscle actin and RT-qPCR for Tenascin C gene expression (N = 4).

Results: Using WB analysis, we observed a decreased total protein abundance of Tenascin C in miR-200b-/- lungs (miR-200b+/+: 3.8 × 107 ± 1 × 107; miR-200b-/-: 1.9 × 107 ± 5 × 106; p = 0.002). Immunofluorescence confirmed decreased total Tenascin C in miR-200b-/- lungs. Tenascin C was significantly decreased in the mesenchyme but relatively increased in the airways of mutant lungs. Total lung RNA expression of Tenascin C was higher in miR-200b-/- lungs.

Conclusion: We report dysregulation of Tenascin C in lungs of miR-200b-/- mice. This suggests that absence of miR-200b results in abnormal Tenascin C abundance contributing to the lung hypoplasia observed in miR-200b-/- mice.

Keywords: Congenital diaphragmatic hernia; Pulmonary hypoplasia; Tenascin C; miR-200b.

cuevana 3

Follow @ Instagram

Translate »

TasteKid.com provides Menu of different Restaurant. Now get all Restaurant Menu, Prices, Catering, Near Me locations, Open Hours. You can quickly find Near me Restaurants around your place.

123 movies soap2day